Pmon530
WebAug 25, 2016 · a pMON530-GFP, used as the blank vector control; b pMON530-SmDXS1-GFP; c pMON530-SmDXS2-GFP. Full size image. PCR and qRT-PCR analysis of transgenic hairy roots. The observed differential expression of the two SmDXS genes suggested that they perform distinct regulatory roles in the first step of the MEP pathway. WebApr 9, 2015 · The pMON530:CaMV35S:ASL3-GFP plasmid was introduced into tobacco cells using Agrobacterium-mediated infection method. Meanwhile, empty GFP vector was used as a control. As a result, the green fluorescent signals of ASL3-GFP fusion protein perfectly overlapped with chloroplast autofluorescence in transformed tobacco mesophyll …
Pmon530
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WebFeb 1, 2014 · The resultant pMON530-SPTCD9-GFP plasmids were introduced into Agrobacterium EHA105. The bacterial suspension was inoculated through the stomata using a 1-mL plastic syringe and by gentle pressure on the lower epidermal surface of 3–4-week-old tobacco ( Nicotiana tabacum) leaves and cultured at 25 °C. WebThe constructed expression vector pMON530-SmERF1L1-GFP. The AP2/ERF transcription factor SmERF1L1 regulates the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
WebMay 15, 2006 · This DNA fragment was cloned into a T-vector (TaKaRa, Japan), verified by sequencing, and inserted into the plant transformation vector pMON530 (Monsanto, USA), downstream to the 35S promoter. WebJul 30, 2024 · The PCR products were cloned into the pMON530-GFP vector, which was transformed into tobacco (Nicotiana tabacum) mesophyll cells through Agrobacterium-mediated infection. Meanwhile, empty pMON530-GFP vector was used as control. The analysis was carried out based on previously described method (Jiang et al. 2014). …
WebAug 16, 2024 · To investigate the subcellular localization of OpWRKY3, its full length of OpWRKY3 was amplified and inserted into vector pMON530-GFP located at the restriction site of Bgl II and Kpn I to generate pMON530-OpWRKY3-GFP. The constructed expression vector was transferred into Agrobacterium strain ASE and injected into 60-day old … WebDec 1, 2016 · The authors are grateful to Prof. Zhongnan Yang (Shanghai Normal University) and Yaoguang Liu (State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University) for kindly providing pMON530-GFP vector, and the pYLgRNA-OsU6 and pYLCRISPR/Cas9-MH) vectors, …
WebApr 27, 2024 · Then, the constructed vector and the pMON530 (used as the control) was transformed into the ASE strain and expressed in tobacco leaves, respectively. In the leaves of control vector transformed plant, the fluorescence of GFP was detected in the cytoplasm and nucleus (Figure Figure3 3). In contrast (to this), the fluorescence of SmWRKY1/GFP ...
WebFeb 15, 2024 · To examine the subcellular localization of SmERF1L1, the full-length ORF of SmERF1L1 was amplified and cloned into pMON530 vector between the BglII and KpnI sites to generate the expression vector pMON530-SmERF1L1-GFP under the control of a constitutive CaMV35S promoter. fixed term employment contractsWebDownload scientific diagram Schematic representation of the T-DNA region of the binary vectors pMON337-TGMV-GFP and pMON530-GFP. fixed term deposit australiaWebAug 16, 2024 · To investigate the subcellular localization of OpWRKY3, its full length of OpWRKY3 was amplified and inserted into vector pMON530-GFP located at the restriction site of Bgl II and Kpn I to generate pMON530-OpWRKY3-GFP. The constructed expression vector was transferred into Agrobacterium strain ASE and injected into 60-day old … can micro calcifications become cancerousWebApr 27, 2024 · To experimentally confirm the subcellular localization of SmWRKY1, SmWRKY1 was cloned into the pMON530 vector to fuse with green fluorescent protein (GFP) reporter gene to generate vector pMON530-SmWRKY1-GFP. fixed term eventuality clauseWebJan 14, 2010 · The resulting plasmid pMON530-CLS was first introduced into Agrobacterium tumefaciens by electroporation and then transformed into the Arabidopsis las mutant by floral dip method (Clough and Bent 1998). Transgenic plants were selected on MS medium containing 20 mg/L Kanamycin. DNA Isolation and Gel Blot Analysis can microchips be recycledWebApr 25, 2000 · 35 S-AtNramp3 (filled bars): homozygous, single insertion line transformed with pMON530 containing AtNramp3 cDNA driven by the 35 S CaMV promoter. To determine whether AtNramp3 affects Fe accumulation, we measured the iron content of control and AtNramp3 overexpressing seedlings grown in minimal medium … fixed term faculty definitionWebJun 1, 1992 · Following electroporation and overnight incubation, the protoplasts were lysed and the extract assayed for GUS activity using the substrate 4-methyl umbelliferyl glucuronide (70, 12). The relative activity of each pro- moter construct was unaffected by coelectroporation with pMON530 DNA without an ACMV ORF insert (not shown). can micro k capsules be opened